Study of Sungkai (Peronema canescens, Jack) Leaf Extract Activity as an Immunostimulators With In vivo and In vitro Methods

Introduction: Sungkai (Peronema canescens, Jack.) contains polysaccharides, terpenoids, alkaloids, and polyphenols which have pharmacological activity as immunostimulants. Objective: This study aimed to see how the effect of extract an immunostimulant agent was carried out in vitro vivo. Materials Methods: conducted using two methods, namely vivo vitro. In research method test phagocytic capacity macrophage cells, percentage leukocytes, total number leukocytes. used 30 male white mice animals that were randomly divided into 5 treatment groups. Each group consisting 6 given different treatments. The negative control with 0.5% NaCMC suspension, substance suspension ethanol various doses 800, 400, 200 mg/kgBW, lastly comparison Stimuno a dose 50 mg/kg orally for 7 days. On day 8, blood taken from mice's vein count its then followed by intraperitoneal injection Staphylococcus aureus bacteria suspension. After 1 hour administration bacterial peritoneal fluid be observed cells. viability immunostimulatory RAW 264.7 cells extraction at concentration 1.10, 100 g/m. cell microtetrazolium (MTT) aims whether sample is safe not toxic observing value should exceed >90%. g/mL found non-toxic Thus, this can performed on LPS induced their levels IL-6 TNF-α. (proinflammatory cytokines) compared alone sandwich ELISA (Enzyme-Linked Immunosorbent Assay) method. Results: observations analyzed one-way ANOVA Duncan's follow-up (significance p<0.05). results showed variations increased significantly (p<0.05) along leukocyte leukocytes had significant increase (p<0.05). It could TNF- tested post hoc test. Conclusion: leaf (Peronemacanescsens of800, mg/kgBW has both